Mass culture of conchocelis filaments in shells
Chul Hyun Sohn

Dept. of Aquaculture, Pukyong National University, Busan 601-737 KOREA

August 30, 2003

Cultivation work

Korean has been long time cultivated seaweeds especially Porphyra Present cultivated species of Porphyra in Korea is P. dentata, P. kuniedae, P. pseudolinearis, P. seriata, P. tenera and P. yezoensis including a hybrid by crossing between two dioecious species, P. pseudolinearis and P. dentata as a new cultivar. The production of Porphyra from culture grounds is estimated to be 168,000 tons (wet wt.) in 2001. Now a good quality of Porphyraproducts is strongly demanded and consequently provides higher prices for the product.

Otherwise maritime cultivation especially fin fish casing cultivation has been rapidly developed and it makes serious self pollution in culture grounds and now newly recognize on that the polyculture with seaweed will be one of the way for environmentally friendly cultivation for the future .

In response to the problem, a joint project was established between Ministry of Maritime Affairs& Fisheries of Korea and National Oceanic &Atmospheric Administration of US.

The shell conchocelis cultivation was carried out on two species, CT-23-1(Pophyra leucosticte) and ME-7-6(Pophyra amplissima) which had been seeding with free-living conchocelis by Dr. Peimin from China. To our knowledge, the strain ME-7-6 was first attempt for shell culture however, unfortunately the information on this strain was very rare not only culture technique but also natural habitat.

Culture tanks were designed about 300L in seawater capacity with cooling water recycling system for temperature control.
The cultivation was progressed according to basically method of shell culture such as controlling temperature, light intensity, photoperiod and water quality etc.

Culture of conchocelis filaments in shells were processed mainly three steps; 1)shell culture for conchocelis growth 2)shell culture for conchosporangial formation 3)conchospore maturation and the seeding method were designed two ways such as conchospore attaching on net and free-living.

Growth and development of concohocellis were monitored every day in BRVAS and UCONN lab.

Samples were observed with microscope in naturally, staining with methylen blue and/or decalcified with Pereny’s solution. Until late May the conchocelis filaments almost cover the shell surface and the shell surface changed to bright pink color. From this time conchocelis culture changed to conchosporangia formation culture and the conchospangium formation started during late May to early Jun.

Although lot of conchosporangis has been continuously formed and increased during last Jun to July and August, however, conchospore is still not matured. On this problem various conditions such as biological, chemical and physical possibility were considered and tried during these culture periods and the sporangia is still developing now.

Other activities (Presentations & Society)

BRVAS (Bridgeport Regional Vocational Aquaculture School) : “Introduction on advanced and newly developed seaweed cultivation in Korea” in Mar. 27, 2003
Working group meeting in BRVAS : “Introduction on advanced and newly developed seaweed cultivation in Korea” in Mar. 30, 2003
NEAS(Northeast Algal Symposium): participation in April 25-27, 2003
PSA(Phycological Society of America):“Developments on two newly cultivated species Capsosiphon fulvescens and Costaria costata in Korea” in Jun 14-18, 2003
NOAA (National Oceanographic Atmospheric Administration):“Advanced seaweed cultivation in Korea” in Jun 26, 2003

Future contact information for obtaining F. chinensis broodstocks from Korea
(Inchon-TaeAn-Yosu-Pusan, Korea)

Dr. In Kwon Jang Senior Research Fellow National Fisheries Research & Development Institute (NFRDI)
West Sea Fisheries Research Institute Tel: +82-032-763-4308
Fax: +82-032-761-0466
Mobile: +82-019-520-3511
Email: jangik@nfrdi.re.kr

Mr. Yeon Jin Kim Manager/owner TaeAn SPF Hatchery Through Dr. Jang
Dr. An Yong Kim Director West Sea Fisheries Research Institute, NFRDI Tel: +82-032-761-3771
Mobile: +82-011-773-3771

Dr. Young-Je Park Division Chief West Sea Fisheries Research Institute, NFRDI Tel: +82-032-764-6632
Fax: +82-032-761-0466
Mobile: +82-011-371-3922
Email: scallop@shinbiro.com

Dr. Hyon-Sob Han West Sea Fisheries Research Institute, NFRDI
Dr. Yoon Kim Director Aquaculture Department, NFRDI Tel: +82-51-720-2400~2
Fax: +82-51-720-2439
Email: yoonkim@nfrdi.re.kr

Dr. Jong Yun Lee Director Biotechnology Division, NFRDI Tel: +82-51-720-2440
Fax: +82-51-720-2456
Email: Leejy@nfrdi.re.kr

Dr. Hyung-Kee Cha Researcher Coastal & Offshore Fisheries Resources Division, NFRDI Tel: +82-051-720-2282
Fax: +82-051-720-2277
Email: hkcha@nfrdi.re.kr

Dr. Jin Yeong Kim Director
Fisheries Scientist Coastal & Offshore Fisheries Resources Division, NFRDI Tel: +82-051-720-2270
Fax: +82-051-720-2277
Email: jiykim@nfrdi.re.kr

Dr. Yong Jin Kang Researcher Aquaculture Division, NFRDI Tel: +82-051-720-2412
Fax: +82-051-720-2439
Email: yikang@nfrdi.re.kr

Dr. Hea-Young Moon Lee Researcher Aquaculture Division, NFRDI Tel: +82-051-720-2423
Fax: +82-051-720-2114
Email: hylee@nfrdi.re.krDr. Jung-Ha Kang Researcher Genetic Engineering Laboratory, Biotechnology Division, NFRDI Tel: +82-051-720-2451
Fax: +82-051-720-2456
Email: kjh12@hotmail.com

Dr. Sung Yun Hong Professor Pukyong National University Tel: +82-051-620-6264
Mobile: +82-017-843-6006
Email: syhong@dolphin.pknu.ac.krDr. Sungchul C. Bai Director

Associate Professor Feeds & Nutrition Research Center
Department of Aquaculture
Pukyong National University Tel: +82-051-620-6874
Fax: +82-051-628-6873
Email: scbai@mail.pknu.ac.krDr. Kyung-Hee Jung Director DMJ Biotech Corp.

DMJ Biotech Research Center Tel: +82-42-862-0365
Fax: +82-42-862-0702
Email: jkh@dmj.orgMiss Jin? Reporter Korean Aquaculture Magazine